thr77 login Options
thr77 login Options
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant purpose in selling mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two further splice variants were being described but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind especially to your goal antibody and block antibody binding. These peptide ordinarily is made up of the epitope regarded from the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to make sure the best possible benefits are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide no longer bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody on your own, you can see which staining is particular.
Target Facts Cyclin E belongs towards the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which contribute into the temporal coordination of each and every mitotic event. Cyclin E kinds a fancy with and features for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress as a result of S section.
The measurement of protein phosphorylation is a get more info great tool for measuring the modulation of receptor activation by both antibodies...
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